News list
Launch of the SafeFood4ClimDiet Project
Published on
15/01/2025
The SafeFood4ClimDiet project, funded by the French National Research Agency (ANR) under the "Jeune Chercheur - Jeune Chercheuse (JCJC)" program, officially kicks off on January 15, 2025.
This pioneering project investigates the effects of climate change on consumer eating habits and associated microbiological risks. Through interdisciplinary approaches, including sociological analysis, text-mining, and risk ranking, it aims to provide actionable recommendations to address current and future food safety challenges.
The project emphasizes raising awareness among consumers and stakeholders about these critical issues.
Stay tuned on our website for updates on SafeFood4ClimDiet!
rtRT-PCR for the detection of O/ME-SA/SA-2018 FMDV
Published on
14/01/2025
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus lineage O/ME-SA/SA-2018, as it is an emerging lineage in South Asia since 2018. The assay has been tested on 34 FMDV positive samples (including 12 SA-2018 samples) with a specificity of 91,7% (11/12 SA-2018 samples detected). The primers and probes are indicated hereafter:
Oligo name
(final concentration)
Sequence (5’-3’)
Use
SA2018_F3 (0.4 μM)
ACAACACCACCAATCCAAC
Forward Primer
SA2018_P3 (0.3 μM)
FAM-ACTCACCCGACTTGCACTGCCGT-TAMRA
Probe
SA2018_Rev2 (0.4 μM)
CGTTGTAAACAGTAGCCATGA
Reverse Primer
The SA-2018 has been validated using Ag-Path kit in a duplex system with β-actin, and following the volumes and concentrations as follow (5 µl of RNA):
Volume (µl)
Concentration
For one tube
Initial
Final
Ultrapure water (DNase RNase Free)
1,15
/
Buffer 2X (kit AgPath-ID™)
12,5
2
1
X
Primer F
1
10
0,4
µM
Primer R
1
10
0,4
µM
Probe FAM-TAMRA
0,75
10
0,3
µM
Primer F β-actine
1
10
0,4
µM
Primer R β-actine
1
10
0,4
µM
Probe VIC-TAMRA β-actine
0,6
5
0,12
µM
RT-PCR mix 25X (Enzyme)
1
25
1
X
Real-time PCR program:
Cycles of RTq-PCR
T°
Time
nb cycles
45°C
10min
1
95°C
10min
1
95°C
15s
45
62°C
1min
NB: This system has been validated on a small number of samples and should therefore be tested against other samples from this lineage.
Outbreak of Foot-and-Mouth disease in Germany
Published on
10/01/2025
An outbreak of Foot-and-Mouth Disease has been reported in Germany in water buffaloes. The outbreak is located in the Brandenburg region, close to Berlin (first reports of this disease in Germany since 1988). This is the first case in the EU since 2011 in Bulgaria. According to the regional authorities, three water buffaloes are confirmed as infected. Restriction zones have been set up around the outbreak. The identification of the serotype is pending.
For more information: https://wahis.woah.org/#/in-review/6177 ; https://www.fli.de/en/news/short-messages/short-message/fli-confirms-foot-and-mouth-disease-in-brandenburg-water-buffalo/
Newsletter December 2024
Published on
18/12/2024
December 2024 Newsletter to the attention of the NRLs for VMPR of Antimicrobials and Dyes in food from animal origin
TRACES 2025: second announcement and call for papers
Published on
17/12/2024
June 3-6, 2025
Residues of pharmacologically active substances in food, feed and environment, within the One Health concept
TRACES 2025 - TRACES
NEW METHODS ONLINE
Published on
17/12/2024
Method for the detection and confirmatory quantification of five nitrofuran metabolite residues in biological matrices using LC-MS/MS
ANSES/LMV/19/01 - V5 of December 2024
And
Method for the detection and quantification of residues of prohibited veterinary medicines in casings using LC-MS/MS
F/CHIM/SM/PTC/036 - V1 of January 2025
2024 - Report - Inter-laboratory test - evaluation of the performance characteristics of pan-lyssavirus real-time RT-PCR
Published on
10/12/2024
We’re glad to inform you that the report of the inter-laboratory test evaluating the performance characteristics of the pan-lyssavirus real-time RT-PCR tests has just been released.
We would like to warmly thank all the participants for their implication and their contribution in a better understanding of the available pan RT-PCR techniques, their variations and implications.
The report is available under restricted access here (onglet ILT reports).
EURCAW-Poultry-SFA’s eighth scientific article
Published on
09/12/2024
The Centre is celebrating the publication of its eighth scientific article as a result of the work carried out in Activity 3 in 2023-2024.
The aim of this study was to assess the performance of latency-to-lie tests without the addition of water, making them more practical for use on farm.
This article named "Research Note: Testing the validity of latency-to-lie tests without water for objective on-farm assessment of walking ability of broiler chickens" has been published in Poultry Science and is available here.
EURCAW Aqua website now available
Published on
21/11/2024
EURCAW Aquatic Animals has their website up and running.
Like the other EURCAWs, Aqua will be posting all of their publications, news and training on their website.
To access the website, visit https://www.eurcaw-aqua.eu/