An inter-laboratory proficiency test is being organized to assess the performance and conformity of laboratories in detecting Paenibacillus larvae and Melissococcus plutonius, the causative agents of American and European foulbrood in honeybees.

Objectives:

To evaluate the accuracy and reliability of results provided by participating laboratories in the detection of these pathogens.

Diagnostic Methods Assessed:

• Microscopy
• Conventional and/or real-time PCR
• Bacterial culture

Test Matrix:

Crushed bee larvae

Provisional Timeline:

• Registration deadline: 30 May 2025
• Sample distribution: June 2025

Eligibility Criteria:

Participation in this PT is mandatory for NRLs  from EU Member States, in accordance with their area of competence. Failure to participate may constitute a breach of regulatory obligations.

Participation by other laboratories, including NRLs from Non-EU or third countries, may be considered; however, it is subject to feasibility and specific conditions.

Please note: Laboratories outside the mandatory participation framework that wish to take part in the external performance evaluation are kindly invited to consult the "PT Presentation Sheet" on the LEILA online platform or contact us at eurl.bee@anses.fr for further information.

Important Note:

Registration is now available through the LEILA online platform, accessible via the following link: https://leila.anses.fr (Reference: PLMP25EU).