EURL
European Union Reference Laboratory for
Foot-and-Mouth disease
LRUE Fièvre Aphteuse
URL:
https://eurl-fmd.anses.fr
Background type:
color
Intro [EN]:
The European Union Reference Laboratory organises Proficiency Tests (PTs) on detection and typing of Foot-and-Mouth disease virus in accordance with the Regulation (EU) No 2017/625.
PT panels for FMDV and SVDV detection are established for virus isolation, RT-PCR, Antigen-ELISA and VP1 sequencing. PT panels for FMD antibody detection are established for FMDV NSP antibodies, FMDV SP antibodies and SVDV antibodies detection by ELISAs or/and virus neutralisation test.
The proficiency test (PT) for FMD virus and SVD virus and antibody detection is organised annually. All EU national reference laboratories (NRLs) and other official laboratories of the network are invited to participate.
Intro [EN]:
The European Union Reference Laboratory yearly organizes a workshop, especially intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The purpose of these workshops is to inform the participants about the activities of the EURL, to present and discuss the results of the PT, to present and discuss scientific research projects and to exchange information on EU legislation, methods and other relevant subjects.
Registration to the next workshopIntro [EN]:
The EURL for FMD, among other missions, gathers data and information on the methods of diagnosis and differential diagnosis used in the different National Laboratories.
A list of methods inspired by the WOAH official methods is available on this website.
Intro [EN]:
The EURL for FMD supports the functions of National Laboratories by storing and supplying reagents and materials for use in diagnosis of foot-and-mouth disease such as virus and/or inactivated antigens, standardised sera, cell lines and other reference reagents.
A list of reagents and material is available on this website.
Display Name:
Foot-and-Mouth disease
Title [FR]:
Méthodes
Title [EN]:
Methods
Title [FR]:
Réactifs disponibles et matériaux de référence
Title [EN]:
Available reagents and reference materials
Methods Background:
Materiau Background:
Code AT Internet:
603911
Nom page d'accueil (AT):
Accueil LRUE FA
Intro [EN]:
The European Union Reference Laboratory organizes each year trainings specifically intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The purpose of these sessions is to implement the further training of experts in laboratory diagnosis with the goal to harmonize the diagnostic techniques at the European and International levels.
FMDV diagnostic training - From 24-28th November 2025
From 18 July 2025 to 01 December 2025
The European Union Reference Laboratory organizes each year trainings specifically intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The 2025 session will run from 24 to 28 November inclusive.
The registration for this session is open.
You will find here the programme for this session and you can register directly on the LRUE website via the link above.
The EURL team is at your disposal for any further information.
WRL - The Pirbright Institute
List of Foot-and-mouth disease reagents available at the WRL the Pirbright Institute can be found here.
FMDV diagnostic training 2026
From 28 April 2026 to 31 December 2026
The European Union Reference Laboratory organizes each year trainings specifically intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The registration for this session is open.
You will find here the programme for Molecular Biology session and Serology session. You can register directly on the LRUE website via the link above.
The EURL team is at your disposal for any further information.
rtRT-PCR for the detection of SAT1/III (“BOT/1/77 like”)
This real-time RT-PCR assay is a molecular tool designed to detect Foot-and-Mouth Disease viruses belonging to the SAT1/III topotype, which have been circulating in the Middle East and the European Union since late 2025. These viruses are closely related to the BOT/1/77 isolate, which is used as a vaccine strain by some companies. At present, this assay is considered a candidate test and has not yet been fully validated; however, it may be useful for the early detection of this specific topotype.
New rt RT-PCR for the detection of the SAT1/III FMDV (candidate assay)
In response to the emergence of SAT1/III in the Middle East and European Union between 2025 and 2026, the following real-time RT-PCR assay (SAT1/III “BOT/1/77 like”) has been tested on few samples from this topotype. Data from EURL from FMD indicate that this assay is able to detect viruses from the SAT/III clade that is actually present in EU and Middle East. At this stage, this is a candidate assay that has not been extensively validated and further work is underway at the EURL and WRL for FMD.
2026 Proficiency test on Foot and Mouth Disease
Test reports:
► Online test report for panel 1,
► Online test report for panel 2,
► Online test report for panel 3,
Beware that the test reports are not linked with Leila. Therefore be careful to keep the link you will get to be able to come back and modify your entries.
Nevertheless once validated you will not be able to modify your test report(s).
Keep in mind that you need to upload the test reports joint in a zip-file in Leila website.
Outbreak of Foot-and-Mouth disease in Greece
An outbreak of Foot-and-Mouth Disease has been reported in Greece (Island of Lesbos) in domestic cattle since 16 March 2026. Identification of serotype is pending.
This is the fifth case within the EU since 2025.
For more information: WAHIS