LRUE Fièvre Aphteuse

URL: 
https://eurl-fmd.anses.fr
Background type: 
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Intro [EN]: 

The European Union Reference Laboratory organises Proficiency Tests (PTs) on detection and typing of Foot-and-Mouth disease virus in accordance with the Regulation (EU) No 2017/625.

PT panels for FMDV and SVDV detection are established for virus isolation, RT-PCR, Antigen-ELISA and VP1 sequencing. PT panels for FMD antibody detection are established for FMDV NSP antibodies, FMDV SP antibodies and SVDV antibodies detection by ELISAs or/and virus neutralisation test.

The proficiency test (PT) for FMD virus and SVD virus and antibody detection is organised annually. All EU national reference laboratories (NRLs) and other official laboratories of the network are invited to participate.

Intro [EN]: 

The European Union Reference Laboratory yearly organizes a workshop, especially intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.

The purpose of these workshops is to inform the participants about the activities of the EURL, to present and discuss the results of the PT, to present and discuss scientific research projects and to exchange information on EU legislation, methods and other relevant subjects.

 

 Registration to the next workshop

Intro [EN]: 

The EURL for FMD, among other missions, gathers data and information on the methods of diagnosis and differential diagnosis used in the different National Laboratories.

A list of methods inspired by the OIE official methods is available on this website.

Intro [EN]: 

The EURL for FMD supports the functions of National Laboratories by storing and supplying reagents and materials for use in diagnosis of foot-and-mouth disease such as virus and/or inactivated antigens, standardised sera, cell lines and other reference reagents.

A list of reagents and material is available on this website.

Display Name: 
Foot-and-Mouth disease
Title [FR]: 
Méthodes
Title [EN]: 
Methods
Title [FR]: 
Réactifs disponibles et matériaux de référence
Title [EN]: 
Available reagents and reference materials
Methods Background: 
Materiau Background: 
Code AT Internet: 
603911
Nom page d'accueil (AT): 
Accueil LRUE FA
Intro [EN]: 

The European Union Reference Laboratory organizes each year trainings specifically intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.

The purpose of these sessions is to implement the further training of experts in laboratory diagnosis with the goal to harmonize the diagnostic techniques at the European and International levels.

Registration to the next training

Scientific meeting of GFRA 2021

The next Scientific meeting of the Global Foot and Mouth disease Research Alliance will be held 1 - 3 November 2021 as a virtual edition due to the COVID-19 pandemic :

https://www.gfra2021.com/home-site/

OIE Terrestrial Manual 2018

 

All methods used for FMD detection, including commercial kits, must be OIE-based methods validated according to international standards.

You will find attached the most recent version of the OIE terrestrial manual chapter regarding foot-and-mouth disease. 

FDMV detection and typing - Decision tree

 

Diagnostic methods for FMD detection and typing are described in the OIE Terrestrial Manual.

This document sums up the diagnostic strategy as a decision tree describing the workflow for implementation of the different methods prescribed. 

All methods used, including commercial kits, must be OIE-based methods validated according to international standards and used successfully in the annual PT.

Samples Treatment

 

This SOP is the OIE-based method currently used at the EURL for FMD.

All other methods used, including commercial kits, must be OIE-based methods validated according to international standards and used successfully in the annual PT.

Virus Isolation

 

This SOP is the OIE-based method currently used at the EURL for FMD detection.

All other methods used, including commercial kits, must be OIE-based methods validated according to international standards and used successfully in the annual PT.

Duplex one-step real-time RT-PCR assays for diagnosis of foot-and-mouth disease

 

This SOP is the OIE-based method currently used at the EURL for FMD detection.

All other methods used, including commercial kits, must be OIE-based methods validated according to international standards and used successfully in the annual PT.

Antigen detection ELISA

 

This SOP is the OIE-based method currently used at the EURL for FMD detection and typing.

All other methods used, including commercial kits, must be OIE-based methods validated according to international standards and used successfully in the annual PT.

Standard operating procedure FMD VP1 amplification

 

This SOP is the OIE-based method currently used at the EURL for FMD detection.

All other methods used, including commercial kits, must be OIE-based methods validated according to international standards and used successfully in the annual PT.

Suspicion of foot-and-mouth disease outbreak in Rodrigues Island

 

For more information : https://promedmail.org/promed-post/?id=8247240

Recent publications

Please, find the recent publications here : https://sitesv2.anses.fr/en/minisite/lrue-fievre-aphteuse/publications

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