The present document describes a standard technique, the High Resolution Melting (HRM) real-time Polymerase Chain Reaction (PCR) to identify the species and five biovars of Brucella in animal / human samples.

High-resolution melting (HRM) is a post-PCR technique that determines with high precision the melt profile of PCR products. A new generation dye is incorporated into a double-stranded DNA. Using a slow constant increase in temperature, fluorescence acquisition allows the distinction between two different populations of amplicons. The method can be used to interrogate small number of SNPs.

Scientific references
Guillaume GIRAULT, Ludivine PERROT, Virginie MICK, Claire PONSART (2022). High-Resolution Melting PCR as Rapid Genotyping Tool for Brucella Species, Microorganisms 10 (336)