This document is applicable to Bison ssp, Bos ssp and Bubalus ssp as listed in the Commission Implementing Regulation (EU) 2018/1882 and describes the guidelines relative to method validation and testing strategy in these species.

Specific guidelines for Bubalus ssp. are in preparation.

According to the Commission Delegated Regulation (EU) 2020/689 Article 6, specific legislation and guidance for brucellosis testing will be made available:

(a) in the websites of the EURL and COM: see Methods | EURL (anses.fr) ;
(b) if no recommendation is provided by EURL websites, OIE Manuals have to be applied
(c) if no recommendation is provided by EURL / OIE websites, Article 34 of Regulation (EU) 2017/625 should be applied:
• National rules. In absence of national rules, relevant methods recommended by NRL validated in accordance with international standards or
• Relevant methods validated with inter or intra laboratory methods validation studies
• In case of urgency, NRL (in absence, other designated lab) may use methods which have not been validated