The present document describes a standard technique aiming at controlling the fulfilment of OIE and EU requirements regarding the standardisation of diagnostic antigens for the detection of antibodies specific of smooth Brucella species (especially B. abortus, B. melitensis and B. suis):
- in animal individual sera by the Rose Bengal Test (RBT) and the Complement Fixation Test (CFT);
- in bovine pooled milk samples by the Milk Ring-Test (MRT).