EURL methods & forms

EURL methods

You will find here the diagnostic methods pursuant to Article 6 of Commission Delegated Regulation (EU) 2020/689.


For the EU listed diseases (pursuant to Commission Implementing Regulation (EU) 2018/1882)

  • Tropilaelaps spp.

Morphological identification of Tropilaelaps spp. (adult form) (OIE method)

Identification of Tropilaelaps spp. using a conventionnal PCR (in-house method) and sequencing


  • Aethina tumida

Morphological identification of the Small Hive Beetle (OIE method)

Identification of the Small Hive Beetle by real time PCR (in-house method)


  • Paenibacillus larvae (causative agent of American foulbrood)

Identification of Paenibacillus larvae using PCR (in-house method)


  • Varroa destructor (agent of varroosis)

see the chapter on varroosis in the OIE Terrestrial Manual


For the other diseases


  • Nosema spp. (causative agents of nosemosis)

Identification and quantification of Nosema spp. by microscopic examination

Identification of Nosema spp. using PCR (in-house method)



  • Melissococcus plutonius (causative agent of European foulbrood)

Identification of Melissococcus plutonius using PCR (in-house method)



  • Multi-viruses

Detection and quantification of the Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Deformed Wing Virus (DWV-A & DWV-B), and Sacbrood Virus (SBV) by real-time RT-PCR

  • Chronic bee paralysis virus

Detection and quantification of the chronic bee paralysis virus (CBPV) by real-time RT-PCR



Test for the presence of the β-actin gene on honeybee samples using PCR (in-house method)


EURL forms

Mite & insect collection form

EURL customer sampling form

EURL reference material request