EURL rabies

URL: 
https://eurl-rabies.anses.fr
Background type: 
color
Intro [EN]: 

Collection of samples, techniques, validation and interpretation of the diagnostic methods for the purposes of rabies surveillance are presented here.

Diagnostic methods considered compliant are presented in the WOAH rabies manual. A list of procedures inspired by the WOAH official methods is also proposed.

Title [EN]: 
Diagnostic methods
Methods Background: 
Materiau Background: 
Code AT Internet: 
624742
Nom page d'accueil (AT): 
Home_EURL_Rabies
Intro [EN]: 

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Protocols other than diagnosis

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Sanger sequencing of lyssaviruses

This protocol describes the preparation of PCR products from lyssavirus-positive samples for the characterization of the lyssavirus species.

English

Detection of Lyssavirus RNA by Real-Time RT-PCR

This protocol describes the generic detection of lyssavirus RNA using Real Time PCR for diagnostic purpose.
English

Detection of Lyssavirus RNA by Conventional RT-PCR

This protocol describes the detection of lyssavirus RNA by conventional hnRT-PCR for diagnostic purpose.

English

Detection of Lyssavirus by the Rabies Tissue Culture Infection Test (RTCIT)

This protocol describes the detection of infectious rabies virus by the RTCIT for diagnostic purpose.

English

Sample treatment for rabies diagnosis

This protocol describes the preparation of brain samples for the detection of lyssavirus antigen (by the FAT) or rabies virus (by the RTCIT) for diagnostic purpose that fulfills the requirements of the ISO/IEC 17025.
English

Biological material packaging and sending of rabies samples

This protocol describes the transport of diagnostic specimens in the context of animal rabies suspicion.

English

Proficiency test for Rabies diagnosis 2021 session

From 31 May 2021 to 28 June 2021

The EURL for rabies is launching a call for participation in a rabies diagnostic proficiency test for 2021.

The test will be performed on a unique panel to evaluate rabies diagnosis conclusion on 10 samples using at least one of the following techniques:

•       FAT (Fluorescent Antibody Test),

•       RTCIT (Rabies Tissue Culture Infection Test)

•       Conventional RT-PCR

•       Real-Time RT-PCR

 

Schedule

Registration must be done before: 4 January 2021

Panels will be sent on: 31 May 2021

Results from participating laboratories must be sent to the EURL for rabies before:  28 June 2021

A report will be issued in: 30 August 2021