♦ 02 March 2015 ♦

In 2013, the EURL initiated a cross-platform evaluation of the PCR performance of ten commercial SYBR Green® kits ( five two-step kits and five one-step kits) using real-time SYBR Green PCR assays (one-step and two-step methods).   We found that the optimised one-step PCR assays had a higher detection sensitivity than the optimised two-step assays regardless of the machine used as well as the pivotal influence of the thermocycler on PCR performance, as well as that of the master mixes.
The results of the comparison of the two types of methods (one-step versus two-step) of the Real Time SYBR Green® RT-PCR were presented in 2013 during the 6th workshop for Rabies NRLs in Athens, Greece: “Comparison of different commercial SYBR Green® RT-PCR kits” and also in 2014 in the 25th International Meeting of Rabies in the Americas (RITA, Cancun, Mexico). See presentation.
The results of this study are now in open access on the BioMed Research International website. See publication